I tried to casually walk down the Pre aisle at SFO a couple weeks ago and the TSA agent correctly assessed my ineligibility. I think it gets encoded into your boarding pass.
It does. Three beeps from the little scanner thingy at the ID check means you have Pre. One beep means you don't.
BTW: the security at SFO is not TSA. It's a TSA approved contractor (Covenant Aviation Security). I think Tampa is also that way.