Well, shell has the '-ne' operator, which is 'not equals' in a terser style.
Really '!=' is just an attempt at rendering '≠' using only ASCII characters. They're a bit like digraphs and trigraphs in C/C++, except everyone is used to them.
I wonder if Unicode is ubiquitous enough now that you could write a language where the real maths operators were used instead. What would that look like?