I was wondering the same thing, given python 2.6 and 3.1, and leafing through the GoF book lately. Exploring that line of inquiry would make for a good article (or two, or more).

It's level of applicability (which I don't think is much to be honest) hasn't really change as Python (the language) has not changed all that much in that time. There's been a handful of syntactic changes (new keywords, exception handling, etc) and some additional protocols (such as with statement), but the fundamentals of the language have not change (dynamically, strongly typed language with first class functions/types).

Design pattern are conceptual implementation strategies and not components, therefore only fundamental language paradigm changes could (should?) make them no longer applicable for the most part.