1. Is the salary enough to pay my rent, feed me, and allow me to save a little bit every month? (This one is obvious. Don't take a job that will put you in debt!!!)
2. Do I trust that my direct manager will protect me from bullshit? (This is a REALLY important one for me, and is the reason I've rejected offers. If your future manager is an asshole or treats you like shit during the interview, don't take the job. A good manager exists to make sure you do the best work you can possibly do for the company, and gives you any resources you might need for that. A good manager is your leader, not your enemy.)
3. Will I be able to learn and grow in this role? (A good manager will help immensely with this one.)
4. Do I like the people I will be working with? (If you don't at least tolerate the people you work with on a daily basis, you're going to be miserable).
5. Is my commute within reason?