Hopefully people will have an open mind reading my comment, however within spiritual circles it is somewhat known that many people can't tolerate doing any type of entheogen too many times; what that amount of times is and dosing where you lose complete connection will be subjective. I'm also not trying to suggest all changes that occur after going through such an experience is the act of "losing your soul or connection to your soul" - however that arguably is possibility at one end of the spectrum of possibilities. Some people seem fine doing Ayahuasca their whole lives, like shamans - whether they are now dependant on it and have regular access to it is another question, however other people through ego mind addiction (entheogens are powerful for breaking physical addictions, however are addictive for the mind) and force themselves into ceremonies or experience that they know they will regularly if not 100% of the time experience pure terror, not listening to their body's fear response for what it doesn't want to experience and is trying to guide them away from experiencing - instead their ego mind maintaining control and tricks them to continue; the body is inherently intelligent and part of the equation that needs to not be ignored to find balance and allowing your body's systems to guide you to optimal health, equilibrium.