Will the presentation be available online anywhere? This topic is very interesting to me as I maintain a Plex server for my family and have always been curious how Netflix does streaming at scale.
I have to say that a lot of this may be overkill for plex. Do you know if plex even uses sendfile?
Truenas also introduced me to ZFS and I have been amazed by it so far! I haven't dug to deep into FreeBSD yet, but that's next on my list.
There's a lot of blogs that go over this in detail for our usecase at home. Your can do it but you will not see much improvements if any at all.
(I'm also curious where the files are sourced from - EC2 would be horrendously expensiveish, but maybe you send to one CDN and then have it propagate the data further outwards (I'm guessing through a mesh topology).)