I teach an intro to networks once or twice a year with a heavy emphasis on Linux networking, and for the last three or four years, I've started the class with a brief primer on file system organization, home directories, and a few other topics that were never an issue for previous groups of students.
I don't think the main obstacle is conceptual as much as helping them understand that folder hierarchies, path variables, and related concepts are important details of the work so that they'll take the time to learn to use and navigate them effectively.