Not layoff-specific, but here are questions [1] you should be asking your manager at every 1:1 meeting:

How is your week going?

What are some of your priorities for this week?

What challenges have you been having?

How can I help you?

What feedback do you have for me?

You will get more information from the answers to these questions than if you were to directly ask your manager "Am I expendable in the next layoff?".

[1] https://workweek.com/2022/09/26/performance-reviews-dont-act...