The martian atmosphere is 2.8% N2. For comparison, Earths atmoshpere is 78.08% N2, while also being more than 100x as dense. And Earth already has soil, and a developed nitrogen cycle, while Mars doesn't.
So there is nowhere near enough Nitrogen in-situ on Mars to support any form of agriculture. It has to be brought from Earth.
> mars has enough gravity to prevent bone loss
https://www.ucsf.edu/news/2017/07/407806/traveling-mars-will...
So yes, lowered gravity has negative impacts on our bones and physiology in general. The gravity on Mars is 1/3 that of Earth. And that's after spending several months in-transit at microgravity.
> But yes, it does mean bringing your own air and being self sufficient, but we do both of those things on the ISS already
The ISS is not self sufficient, and relies on continuos supply runs from earth: https://en.wikipedia.org/wiki/Uncrewed_spaceflights_to_the_I...