Curious why you do CRISPR when HR works great in yeast.
If you are having trouble transforming, try spheroplasty.
If you are having trouble transforming, try spheroplasty.
Same answer for electroporation vs spheroplasty. I’ve found with wild yeasts or less tamed yeasts (pichia), sometimes just nuking the damn thing with kV will just work, whereas those chemical methods can be way more finicky. Time is money