The line doesn't exists. It's most profitable for the company to repeatedly:
1. Have an ad-supported tier and a no-ads tier
2. Wait for most people to move to the no-ads tier
3. Introduce ads into the no-ads tier
4. Wait for people to get sick of ads
5. Introduce a new, higher cost no ads tier
There is no threshold where it's more profitable for the company to never introduce ads at that price point.