My very unscientific test (just running the test suite on the Django project I happen to have up at the moment) proved to be very slightly slower with it installed: 11.2s - 11.3s without Pyston, 11.7s - 11.8s with Pyston.
Realistic loads might have the same routines run more intensely and might see more benefit from the JIT compiler.
Is this project public? I'd love to investigate