The board mentioned above uses PCIe, as does the even smaller alternative “Raspberry Pi Compute Module 4 IoT Router Carrier Board Mini” from DFRobot [1].
Other boards use USB 3, like the “Dual Gigabit Ethernet NICs Carrier Board for Raspberry Pi Compute Module 4” from SeedStudio [2].
I wouldn’t recommend using USB.
[1]: https://www.dfrobot.com/product-2242.html
[2]: https://www.seeedstudio.com/Rapberry-Pi-CM4-Dual-GbE-Carrier...