> Mice were exposed to physical restraint stress as previously described (https://www.nature.com/articles/s41586-020-1935-3). Briefly, female mice were restrained in individual, homemade 50 mL Falcon tubes with air holes for 2 hours per day. Mice could move backward and forward slightly in the restraining tube.

I wonder why they only used female mice. In the original methods study about the effect of stress on hair loss both male and female mice are used.