Yes, absolutely. This is standard practice among my peers in creative writing; the last step of editing is to read your piece aloud (to the cat, or the dog, or to the mirror, or a human victim, or just to yourself), slowly and carefully.
My friend who is finishing up his novel is currently in the reading-aloud stage. He's had various friends in and out of his house to listen to chapters as he does it, too.
As for myself, I've been caught by my friends standing in back stairwells reading papers aloud thirty minutes before class more times than I care to admit.